Sample name: H9_PRO-seq_2

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name H9_PRO-seq_2
sample_desc H9 PRO-seq
treatment DMSO
replicate 2
toggle 1
protocol PRO
organism human
read_type PAIRED
cell_type H9
purpose low pause index; differential analysis
umi_status true_8
umi_length 8
data_source SRA
read1 /project/shefflab/data/guertin/fastq/H9_DMSO_rep2_PE1.fastq.gz
read2 /project/shefflab/data/guertin/fastq/H9_DMSO_rep2_PE2.fastq.gz
srr H9_DMSO_rep2
Sample_title H9_DMSO_rep2
Sample_geo_accession GSM4214081
Sample_source_name_ch1 H9 cells
File_mb 2754.18
Read_type paired
Genome hg38
Raw_reads 115714026
Fastq_reads 115714026
Reads_with_adapter 45847892.0
Uninformative_adapter_reads 28610139.0
Duplicate_reads 3047417.0
Pct_uninformative_adapter_reads 49.4497
Trimmed_reads 56658510
Trim_loss_rate 51.04
Peak_adapter_insertion_size 20
Degradation_ratio 1.0321
Aligned_reads_human_rDNA 5827176.0
Alignment_rate_human_rDNA 10.28
Mapped_reads 43719896
QC_filtered_reads 25554169
Aligned_reads 18165727.0
Alignment_rate 32.06
Total_efficiency 15.7
Read_depth 3.11
Mitochondrial_reads 840435
Maximum_read_length 38
Genome_size 3099922541
Frac_exp_unique_at_10M 0.854
NRF 1.0
PBC1 9741100.0
PBC2 9741100.0
Unmapped_reads 6664276
TSS_coding_score 33.8
TSS_non-coding_score 12.3
Pause_index 41.45
Plus_FRiP 0.39
Minus_FRiP 0.36
mRNA_contamination 1.29
Time 3:45:40
Success 02-27-13:11:12

H9_PRO-seq_2 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 Library complexity
Fig.2 TSS enrichment
Fig.3 Pause index
Fig.4 cFRiF
Fig.5 FRiF
Fig.6 mRNA contamination