FastQCFastQC Report
Thu 27 Feb 2020
H9_PRO-seq_2_R2_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_2_R2_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30855836
Sequences flagged as poor quality0
Sequence length10-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNN8754812.837327110501884No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC7104952.302627613136134No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTT720120.23338210638661677No Hit
CGCGGGCCCAGAAGCGCAGCGACAGCCTC539380.17480647745210987No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN401940.13026385024862072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)