FastQCFastQC Report
Thu 27 Feb 2020
H9_PRO-seq_2_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_2_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28329255
Sequences flagged as poor quality0
Sequence length10-38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3275481.1562181921127117No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN2143790.7567406908512067No Hit
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA1241660.4382960300226745No Hit
GGCACCCGAGAATTCCA1214920.4288570243022628Illumina Small RNA Adapter 2 (100% over 17bp)
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT979050.34559680443414414No Hit
GCACCCGAGAATTCCA758430.2677197123609499Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA660330.2330911984801577Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA582640.2056672510449004Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA507540.17915755285481388Illumina Small RNA Adapter 2 (100% over 14bp)
CGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT355510.12549218113924987No Hit
CCCGAGAATTCCA321200.11338102608063642Illumina Small RNA Adapter 2 (100% over 13bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)