Sample name: H9_PRO-seq_3

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name H9_PRO-seq_3
sample_desc H9 PRO-seq
treatment DMSO
replicate 3
toggle 1
protocol PRO
organism human
read_type PAIRED
cell_type H9
purpose low pause index; differential analysis
umi_status true_8
umi_length 8
data_source SRA
read1 /project/shefflab/data/guertin/fastq/H9_DMSO_rep3_PE1.fastq.gz
read2 /project/shefflab/data/guertin/fastq/H9_DMSO_rep3_PE2.fastq.gz
srr H9_DMSO_rep3
Sample_title H9_DMSO_rep3
Sample_geo_accession GSM4214082
Sample_source_name_ch1 H9 cells
File_mb 2340.86
Read_type paired
Genome hg38
Raw_reads 92628962
Fastq_reads 92628962
Reads_with_adapter 34725419.0
Uninformative_adapter_reads 18106301.0
Duplicate_reads 1862033.0
Pct_uninformative_adapter_reads 39.0943
Trimmed_reads 54838340
Trim_loss_rate 40.8
Peak_adapter_insertion_size 20
Degradation_ratio 0.9769
Aligned_reads_human_rDNA 5405874.0
Alignment_rate_human_rDNA 9.86
Mapped_reads 43295089
QC_filtered_reads 25127415
Aligned_reads 18167674.5
Alignment_rate 33.13
Total_efficiency 19.61
Read_depth 2.98
Mitochondrial_reads 711009
Maximum_read_length 38
Genome_size 3099922541
Frac_exp_unique_at_10M 0.8825
NRF 1.0
PBC1 9740520.5
PBC2 9740520.5
Unmapped_reads 5843431
TSS_coding_score 28.6
TSS_non-coding_score 10.5
Pause_index 33.89
Plus_FRiP 0.39
Minus_FRiP 0.36
mRNA_contamination 1.28
Time 3:36:20
Success 02-27-13:02:22

H9_PRO-seq_3 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 Library complexity
Fig.2 TSS enrichment
Fig.3 Pause index
Fig.4 cFRiF
Fig.5 FRiF
Fig.6 mRNA contamination