FastQCFastQC Report
Thu 27 Feb 2020
H9_PRO-seq_3_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH9_PRO-seq_3_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27419170
Sequences flagged as poor quality0
Sequence length10-38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2667880.9729980885635852No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNN2023760.7380821520126247No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGT819210.2987727199619828No Hit
GGCACCCGAGAATTCCA773320.2820362541973371Illumina Small RNA Adapter 2 (100% over 17bp)
CAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTA652460.23795760411420186No Hit
GCACCCGAGAATTCCA439350.1602346095815446Illumina Small RNA Adapter 2 (100% over 16bp)
CGAGAATTCCA346030.12620002720724224Illumina Small RNA Adapter 2 (100% over 11bp)
CCGAGAATTCCA315670.11512748197702556Illumina Small RNA Adapter 2 (100% over 12bp)
ACCCGAGAATTCCA299150.10910250018508949Illumina Small RNA Adapter 2 (100% over 14bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)