FastQCFastQC Report
Thu 27 Feb 2020
HelaS3_GRO-seq_R1_processed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHelaS3_GRO-seq_R1_processed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72718941
Sequences flagged as poor quality0
Sequence length30-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAG8301011.1415196489178796No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA6384340.8779473287434151No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA5995960.8245389602139558No Hit
AAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAG3019550.4152356949202547No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2437770.33523177957170747No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1906300.2621462818057265No Hit
AGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC1630530.22422356233158017No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATC1627000.22373813172004253No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATC1538720.21159824095898205No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGG1462500.20111679019088027No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCG1359390.1869375407983458No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT1149540.15807985982634154No Hit
ATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC1095920.15070626509811247No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA1033180.14207852669361618No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGG1013690.1393983446486109No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCT988130.1358834419769672No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAG954950.13132066926002126No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGC930110.12790477793124078No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA927920.12760361843003187No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACA881390.1212050103974974No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATA859790.11823467011160133No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCC844380.11611555234282084No Hit
TAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTA834830.11480227689234362No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC808780.11121999150125138No Hit
AGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGT783920.10780134985739136No Hit
ATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGG753720.10364837408729591No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC732850.1007784203018028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)