Sample name: K562_RNA-seq_90

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_90
sample_desc 10% K562 PRO-seq + 90% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_90pct_RNArc_r2.fastq.gz
srr K562_90pct_RNArc_r2
Sample_title K562 RNA spike-in 90pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 779.46
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 4024168.0
Uninformative_adapter_reads 24881.0
Pct_uninformative_adapter_reads 0.2488
Trimmed_reads 9975119
Trim_loss_rate 0.25
Peak_adapter_insertion_size 34
Degradation_ratio 0.23
Aligned_reads_human_rDNA 313360.0
Alignment_rate_human_rDNA 3.14
Mapped_reads 8305810
QC_filtered_reads 1759025
Aligned_reads 6546785
Alignment_rate 65.63
Total_efficiency 65.47
Read_depth 3.75
Mitochondrial_reads 560951
Maximum_read_length 100
Genome_size 3099922541
NRF 0.76
PBC1 0.91
PBC2 18.23
Unmapped_reads 1355949
TSS_coding_score 18.6
TSS_non-coding_score 3.2
Pause_index 12.14
Plus_FRiP 0.4
Minus_FRiP 0.4
mRNA_contamination 10.06
Time 1:02:06
Success 02-27-10:26:37

K562_RNA-seq_90 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination