Sample name: K562_RNA-seq_80

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_80
sample_desc 20% K562 PRO-seq + 80% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_80pct_RNArc_r2.fastq.gz
srr K562_80pct_RNArc_r2
Sample_title K562 RNA spike-in 80pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 784.39
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 4521696.0
Uninformative_adapter_reads 49637.0
Pct_uninformative_adapter_reads 0.4964
Trimmed_reads 9950363
Trim_loss_rate 0.5
Peak_adapter_insertion_size 34
Degradation_ratio 0.2303
Aligned_reads_human_rDNA 378416.0
Alignment_rate_human_rDNA 3.8
Mapped_reads 8355005
QC_filtered_reads 1668539
Aligned_reads 6686466
Alignment_rate 67.2
Total_efficiency 66.86
Read_depth 3.21
Mitochondrial_reads 519002
Maximum_read_length 100
Genome_size 3099922541
NRF 0.8
PBC1 0.93
PBC2 22.13
Unmapped_reads 1216942
TSS_coding_score 18.1
TSS_non-coding_score 3.6
Pause_index 12.25
Plus_FRiP 0.39
Minus_FRiP 0.39
mRNA_contamination 8.02
Time 1:03:13
Success 02-27-10:27:16

K562_RNA-seq_80 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination