Sample name: K562_RNA-seq_70

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_70
sample_desc 30% K562 PRO-seq + 70% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_70pct_RNArc_r2.fastq.gz
srr K562_70pct_RNArc_r2
Sample_title K562 RNA spike-in 70pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 788.96
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 5020699.0
Uninformative_adapter_reads 74592.0
Pct_uninformative_adapter_reads 0.7459
Trimmed_reads 9925408
Trim_loss_rate 0.75
Peak_adapter_insertion_size 34
Degradation_ratio 0.2307
Aligned_reads_human_rDNA 443714.0
Alignment_rate_human_rDNA 4.47
Mapped_reads 8403304
QC_filtered_reads 1577377
Aligned_reads 6825927
Alignment_rate 68.77
Total_efficiency 68.26
Read_depth 2.86
Mitochondrial_reads 477155
Maximum_read_length 100
Genome_size 3099922541
NRF 0.83
PBC1 0.94
PBC2 26.62
Unmapped_reads 1078390
TSS_coding_score 17.6
TSS_non-coding_score 3.9
Pause_index 12.41
Plus_FRiP 0.39
Minus_FRiP 0.38
mRNA_contamination 6.57
Time 1:03:23
Success 02-27-10:27:24

K562_RNA-seq_70 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination