Sample name: K562_RNA-seq_60

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_60
sample_desc 40% K562 PRO-seq + 60% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_60pct_RNArc_r2.fastq.gz
srr K562_60pct_RNArc_r2
Sample_title K562 RNA spike-in 60pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 793.14
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 5519636.0
Uninformative_adapter_reads 99594.0
Pct_uninformative_adapter_reads 0.9959
Trimmed_reads 9900406
Trim_loss_rate 1.0
Peak_adapter_insertion_size 34
Degradation_ratio 0.2307
Aligned_reads_human_rDNA 508442.0
Alignment_rate_human_rDNA 5.14
Mapped_reads 8452082
QC_filtered_reads 1486236
Aligned_reads 6965846
Alignment_rate 70.36
Total_efficiency 69.66
Read_depth 2.6
Mitochondrial_reads 434938
Maximum_read_length 100
Genome_size 3099922541
NRF 0.86
PBC1 0.95
PBC2 31.45
Unmapped_reads 939882
TSS_coding_score 17.1
TSS_non-coding_score 4.3
Pause_index 12.56
Plus_FRiP 0.38
Minus_FRiP 0.38
mRNA_contamination 5.45
Time 1:04:08
Success 02-27-10:28:10

K562_RNA-seq_60 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination