Sample name: K562_RNA-seq_50

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_50
sample_desc 50% K562 PRO-seq + 50% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_50pct_RNArc_r2.fastq.gz
srr K562_50pct_RNArc_r2
Sample_title K562 RNA spike-in 50pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 796.96
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 6017590.0
Uninformative_adapter_reads 124631.0
Pct_uninformative_adapter_reads 1.2463
Trimmed_reads 9875369
Trim_loss_rate 1.25
Peak_adapter_insertion_size 34
Degradation_ratio 0.2304
Aligned_reads_human_rDNA 573409.0
Alignment_rate_human_rDNA 5.81
Mapped_reads 8499876
QC_filtered_reads 1395808
Aligned_reads 7104068
Alignment_rate 71.94
Total_efficiency 71.04
Read_depth 2.41
Mitochondrial_reads 393154
Maximum_read_length 100
Genome_size 3099922541
NRF 0.89
PBC1 0.96
PBC2 36.68
Unmapped_reads 802084
TSS_coding_score 17.0
TSS_non-coding_score 4.7
Pause_index 12.83
Plus_FRiP 0.38
Minus_FRiP 0.37
mRNA_contamination 4.6
Time 1:05:47
Success 02-27-10:29:22

K562_RNA-seq_50 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination