Sample name: K562_RNA-seq_40

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_40
sample_desc 60% K562 PRO-seq + 40% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_40pct_RNArc_r2.fastq.gz
srr K562_40pct_RNArc_r2
Sample_title K562 RNA spike-in 40pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 800.5
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 6515223.0
Uninformative_adapter_reads 149598.0
Pct_uninformative_adapter_reads 1.496
Trimmed_reads 9850402
Trim_loss_rate 1.5
Peak_adapter_insertion_size 34
Degradation_ratio 0.2301
Aligned_reads_human_rDNA 638008.0
Alignment_rate_human_rDNA 6.48
Mapped_reads 8549090
QC_filtered_reads 1305366
Aligned_reads 7243724
Alignment_rate 73.54
Total_efficiency 72.44
Read_depth 2.26
Mitochondrial_reads 351192
Maximum_read_length 100
Genome_size 3099922541
NRF 0.91
PBC1 0.97
PBC2 41.7
Unmapped_reads 663304
TSS_coding_score 16.7
TSS_non-coding_score 5.0
Pause_index 12.91
Plus_FRiP 0.37
Minus_FRiP 0.36
mRNA_contamination 3.83
Time 1:13:24
Success 02-27-10:36:56

K562_RNA-seq_40 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination