Sample name: K562_RNA-seq_30

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_30
sample_desc 70% K562 PRO-seq + 30% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_30pct_RNArc_r2.fastq.gz
srr K562_30pct_RNArc_r2
Sample_title K562 RNA spike-in 30pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 803.62
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 7013383.0
Uninformative_adapter_reads 174726.0
Pct_uninformative_adapter_reads 1.7473
Trimmed_reads 9825274
Trim_loss_rate 1.75
Peak_adapter_insertion_size 34
Degradation_ratio 0.2303
Aligned_reads_human_rDNA 703191.0
Alignment_rate_human_rDNA 7.16
Mapped_reads 8597206
QC_filtered_reads 1214446
Aligned_reads 7382760
Alignment_rate 75.14
Total_efficiency 73.83
Read_depth 2.15
Mitochondrial_reads 309258
Maximum_read_length 100
Genome_size 3099922541
NRF 0.93
PBC1 0.97
PBC2 46.16
Unmapped_reads 524877
TSS_coding_score 16.2
TSS_non-coding_score 5.3
Pause_index 13.1
Plus_FRiP 0.37
Minus_FRiP 0.36
mRNA_contamination 3.21
Time 0:57:50
Success 02-27-10:21:26

K562_RNA-seq_30 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination