Sample name: K562_RNA-seq_20

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_20
sample_desc 80% K562 PRO-seq + 20% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_20pct_RNArc_r2.fastq.gz
srr K562_20pct_RNArc_r2
Sample_title K562 RNA spike-in 20pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 806.51
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 7511497.0
Uninformative_adapter_reads 199835.0
Pct_uninformative_adapter_reads 1.9984
Trimmed_reads 9800165
Trim_loss_rate 2.0
Peak_adapter_insertion_size 34
Degradation_ratio 0.2305
Aligned_reads_human_rDNA 768600.0
Alignment_rate_human_rDNA 7.84
Mapped_reads 8644667
QC_filtered_reads 1123750
Aligned_reads 7520917
Alignment_rate 76.74
Total_efficiency 75.21
Read_depth 2.05
Mitochondrial_reads 267632
Maximum_read_length 100
Genome_size 3099922541
NRF 0.94
PBC1 0.97
PBC2 49.21
Unmapped_reads 386898
TSS_coding_score 15.9
TSS_non-coding_score 5.3
Pause_index 13.46
Plus_FRiP 0.36
Minus_FRiP 0.35
mRNA_contamination 2.63
Time 1:01:28
Success 02-27-10:24:31

K562_RNA-seq_20 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination