Sample name: K562_RNA-seq_10

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name K562_RNA-seq_10
sample_desc 90% K562 PRO-seq + 10% K562 RNA-seq
treatment none
replicate 1
toggle 1
protocol PRO
organism human
read_type SINGLE
cell_type K562
purpose mRNA contamination; FRiF/PRiF
umi_status FALSE
umi_length 0
data_source SRA
read1 /project/shefflab/data/guertin/fastq/K562_10pct_RNArc_r2.fastq.gz
srr K562_10pct_RNArc_r2
Sample_title K562 RNA spike-in 10pct RC
Sample_geo_accession GSM1480327;GSM765405
Sample_source_name_ch1 K562 cells
File_mb 806.57
Read_type single
Genome hg38
Raw_reads 10000000
Fastq_reads 10000000
Reads_with_adapter 8011909.0
Uninformative_adapter_reads 224478.0
Pct_uninformative_adapter_reads 2.2448
Trimmed_reads 9775522
Trim_loss_rate 2.24
Peak_adapter_insertion_size 34
Degradation_ratio 0.2306
Aligned_reads_human_rDNA 833666.0
Alignment_rate_human_rDNA 8.53
Mapped_reads 8700764
QC_filtered_reads 1036653
Aligned_reads 7664111
Alignment_rate 78.4
Total_efficiency 76.64
Read_depth 1.98
Mitochondrial_reads 227220
Maximum_read_length 100
Genome_size 3099922541
NRF 0.95
PBC1 0.98
PBC2 50.04
Unmapped_reads 241092
TSS_coding_score 15.6
TSS_non-coding_score 5.6
Pause_index 13.88
Plus_FRiP 0.36
Minus_FRiP 0.34
mRNA_contamination 2.06
Time 1:02:06
Success 02-27-10:25:08

K562_RNA-seq_10 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 TSS enrichment
Fig.2 Pause index
Fig.3 cFRiF
Fig.4 FRiF
Fig.5 mRNA contamination