Sample name: HEK_PRO-seq

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name HEK_PRO-seq
sample_desc HEK w/ osTIR1, ZNF143AID PRO-seq
treatment Auxin
replicate 1
toggle 1
protocol PRO
organism human
read_type PAIRED
cell_type HEK293T
purpose very intact RNA
umi_status true_8
umi_length 8
data_source SRA
read1 /project/shefflab/data/guertin/fastq/SRR8608074_PE1.fastq.gz
read2 /project/shefflab/data/guertin/fastq/SRR8608074_PE2.fastq.gz
srr SRR8608074
srx SRX5407488
Sample_title HEK_TIR1_ZNF143AID_Auxin_rep4
Sample_geo_accession GSM3618147
Sample_source_name_ch1 HEK-293T cells
Sample_characteristics_ch1 cell line: HEK-293T
gsm_id GSM3618147
File_mb 1337.94
Read_type paired
Genome hg38
Raw_reads 51533198
Fastq_reads 51533198
Reads_with_adapter 16626152.0
Uninformative_adapter_reads 5380555.0
Duplicate_reads 916082.0
Pct_uninformative_adapter_reads 20.8819
Trimmed_reads 40207164
Trim_loss_rate 21.98
Peak_adapter_insertion_size 37
Degradation_ratio 0.5783
Aligned_reads_human_rDNA 4230222.0
Alignment_rate_human_rDNA 10.52
Mapped_reads 33104378
QC_filtered_reads 18769818
Aligned_reads 14334559.5
Alignment_rate 35.65
Total_efficiency 27.82
Read_depth 2.41
Mitochondrial_reads 325556
Maximum_read_length 38
Genome_size 3099922541
Frac_exp_unique_at_10M 0.9097
NRF 1.0
PBC1 7591734.5
PBC2 7591734.5
Unmapped_reads 2807990
TSS_coding_score 16.0
TSS_non-coding_score 2.4
Pause_index 14.83
Plus_FRiP 0.39
Minus_FRiP 0.37
mRNA_contamination 1.25
Time 2:50:30
Success 02-27-12:15:32

HEK_PRO-seq objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 Library complexity
Fig.2 TSS enrichment
Fig.3 Pause index
Fig.4 cFRiF
Fig.5 FRiF
Fig.6 mRNA contamination