Sample name: H9_treated_PRO-seq_3

Log file Pipeline profile Pipeline commands Stats summary file


Looper stats summary

sample_name H9_treated_PRO-seq_3
sample_desc H9 treated PRO-seq
treatment 200 nM romidepsin
replicate 3
toggle 1
protocol PRO
organism human
read_type PAIRED
cell_type H9
purpose high pause index; differential analysis
umi_status true_8
umi_length 8
data_source SRA
read1 /project/shefflab/data/guertin/fastq/H9_200nM_romidepsin_...
read2 /project/shefflab/data/guertin/fastq/H9_200nM_romidepsin_...
srr H9_200nM_romidepsin_rep3
Sample_title H9_200nM_romidepsin_rep3
Sample_geo_accession GSM4214085
Sample_source_name_ch1 H9 cells
File_mb 2905.11
Read_type paired
Genome hg38
Raw_reads 120377388
Fastq_reads 120377388
Reads_with_adapter 46004533.0
Uninformative_adapter_reads 26667722.0
Duplicate_reads 4932446.0
Pct_uninformative_adapter_reads 44.3069
Trimmed_reads 64640918
Trim_loss_rate 46.3
Peak_adapter_insertion_size 20
Degradation_ratio 0.9116
Aligned_reads_human_rDNA 6696846.0
Alignment_rate_human_rDNA 10.36
Mapped_reads 50176577
QC_filtered_reads 29080016
Aligned_reads 21096561.0
Alignment_rate 32.64
Total_efficiency 17.53
Read_depth 3.98
Mitochondrial_reads 667197
Maximum_read_length 38
Genome_size 3099922541
Frac_exp_unique_at_10M 0.7916
NRF 1.0
PBC1 11412874.5
PBC2 11412874.5
Unmapped_reads 7400125
TSS_coding_score 60.1
TSS_non-coding_score 17.8
Pause_index 74.17
Plus_FRiP 0.4
Minus_FRiP 0.38
mRNA_contamination 1.1
Time 3:27:30
Success 02-27-12:54:05

H9_treated_PRO-seq_3 objects

Links
Figures
Fig.0 Adapter insertion distribution
Fig.1 Library complexity
Fig.2 TSS enrichment
Fig.3 Pause index
Fig.4 cFRiF
Fig.5 FRiF
Fig.6 mRNA contamination